Toluidine Blue is a normal thiazine metachromatic dye with very high similitude to acidic components and thereby affecting/staining the tissues which are rich in DNA and RNA. Because of this feature it have a wide number of applications both in living tissues and a different and special stain owing to its metachromatic property. In vivo, toluidine Blue has been used to identify dysplasia and carcinoma produced in the oral cavity. The components such as mast cells granules, cartilage and mucins are treated with toluidine blue with a aim of highlighting them. In this article I have shared how Dr Devicharan Shetty have explained the implement of toluidine blue used as a dye to detect marginal leakage. So it is a mix of its chemistry , the technique used in the process and the various applications of toluidine blue.
Toluidine is known by all because of its various medical applications. It was discovered by William Henry Perkin in 1856. After its discovery it is has been popularly used in the dye industry. Toluidine Blue is also known as methylaniline or aminotoluene. ortho-toluidine, para-toluidine, and meta-toluidine are basically the three isoforms of toluidine blue. It is majorly used in mucosal lesions as a vital stain. Also it is majorly used in as applications in the tissue section to highlight the stain according to its metachromatic property.
I hope you are clear about what is vital staining,It is the staining appeared in the cells or tissues when they are in the living state. The two techniques involved in the vital staining are the intravital staining and the supravital staining. The intravital staining is basically the staining inside the living body(in vivo) while the supravital staining is applied outside the body t slide preparation of the detached cell. Toluidine Blue was first applied in vivo by reichart in 1963 for uterine cervical carcinoma.
Toluidine Blue is very effective in detecting relative differences between normal and malignant cells and tissues . Also it is very useful in highlighting the vital stain of malignant oral lesions. Also it is very helpful in identification of early lesions which are being missed in normal clinical examinations. Most of the dyes stains on the tissue with the same colour with different degrees of intensity. The reaction of the staining is is called as metachromasy and the tissue showcase metachromasia. There are other tissues components such as mucin, cartilage and mast cells granules which have exhibited the metachromasia. Toluidine blue is one dye which is being used in the tissues section for the identification of the various cells.